Botanical Extract, a non-intoxicating component of the cannabis plant, has generated significant interest among scientists and physicians in recent years–but how botanical extract exerts its therapeutic impact on a molecular level is still being sorted out. botanical extract oil is a pleiotropic drug in that it produces many effects through multiple molecular pathways. The scientific literature has identified more than 65 molecular targets of botanical extract.
Although botanical extract has little binding affinity for either of the two cannabinoid receptors (CB1and CB2), botanical extract oil modulates several non-cannabinoid receptors and ion channels. Botanical extract also acts through various receptor-independent pathways–for example, by delaying the “reuptake” of endogenous neurotransmitters (such as anandamide and adenosine) and by enhancing or inhibiting the binding action of certain G-protein coupled receptors.
Here are some of the ways that botanical extract confers its manifold therapeutic effects.
Jose Alexandre Crippa and his colleagues at the University of San Paulo in Brazil and King’s College in London have conducted pioneering research into botanical extract and the neural correlates of anxiety. At high concentrations, botanical extract directly activates the 5-HT1A (hydroxytryptamine) serotonin receptor, thereby conferring an anti-anxiety effect. This G-coupled protein receptor is implicated in a range of biological and neurological processes, including (but not limited to) anxiety, addiction, appetite, sleep, pain perception, nausea and vomiting.
5-HT1A is a member of the family of 5-HT receptors, which are activated by the neurotransmitter serotonin. Found in both the central and peripheral nervous systems, 5-HT receptors trigger various intracellular cascades of chemical messages to produce either an excitatory or inhibitory response, depending on the chemical context of the message.
[Cannabidiolic acid], the raw, unheated version of botanical extract that is present in the cannabis plant, also has a strong affinity for the 5-HT1A receptor (even more so than botanical extract). Preclinical studies indicate that botanical extract acid is a potent anti-emetic, stronger than either botanical extract or THC, which also have anti-nausea properties.
Botanical extract directly interacts with various ion channels to confer a therapeutic effect. Botanical extract, for example, binds to TRPV1 receptors, which also function as ion channels. TRPV1 is known to mediate pain perception, inflammation and body temperature.
TRPV is the technical abbreviation for “transient receptor potential cation channel subfamily V.” TRPV1 is one of several dozen TRP (pronounced “trip”) receptor variants or subfamilies that mediate the effects of a wide range of medicinal herbs.Scientists also refer to TRPV1 as a “vanilloid receptor,” named after the flavorful vanilla bean. Vanilla contains eugenol, an essential oil that has antiseptic and analgesic properties; it also helps to unclog blood vessels. Historically, the vanilla bean has been used as a folk cure for headaches.
Botanical extract binds to TRPV1, which can influence pain perception. Capsaicin–the pungent compound in hot chili peppers–activates the TRVP1 receptor. Anandamide, the endogenous cannabinoid, is also a TRPV1 agonist.
Whereas botanical extract oil directly activates the 5-HT1A serotonin receptor and several TRPV ion channels, some studies indicate that botanical extract functions as an antagonist that blocks, or deactivates, another G protein-coupled receptor known as GPR55.
GPR55 has been dubbed an “orphan receptor” because scientists are still not sure if it belongs to a larger family of receptors. GPR55 is widely expressed in the brain, especially in the cerebellum. It is involved in modulating blood pressure and bone density, among other physiological processes.
GPR55 promotes osteoclast cell function, which facilitates bone reabsorption. Overactive GPR55 receptor signaling is associated with osteoporosis.GPR55, when activated, also promotes cancer cell proliferation, according to a 2010 study by researchers at the Chinese Academy of Sciences in Shanghai. This receptor is expressed in various types of cancer.
Botanical extract is a GPR55 antagonist, as University of Aberdeen scientist Ruth Ross disclosed at the 2010 conference of the International Cannabinoid Research Society in Lund, Sweden. By blocking GPR55 signaling, Botanical extract may act to decrease both bone reabsorption and cancer cell proliferation.
PPARS - NUCLEAR RECEPTORS
Botanical extract also exerts an anti-cancer effect by activating PPARs [peroxisome proliferator activated receptors] that are situated on the surface of the cell’s nucleus. Activation of the receptor known as PPAR-gamma has an anti-proliferative effect as well as an ability to induce tumor regression in human lung cancer cell lines. PPAR-gamma activation degrades amyloid-beta plaque, a key molecule linked to the development of Alzheimer’s disease. This is one of the reasons why botanical extract oil, a PPAR-gamma agonist, may be a useful remedy for Alzheimer’s patients.
PPAR receptors also regulate genes that are involved in energy homeostasis, lipid uptake, insulin sensitivity, and other metabolic functions. Diabetics, accordingly, may benefit from a botanical extract-rich treatment regimen.
BOTANICAL EXTRACT AS A REUPTAKE INHIBITOR
How does botanical extract, an exogenous plant compound, get inside a human cell to bind to a nuclear receptor? First it has to pass through the cell membrane by hitching a ride with a fatty acid binding protein (FABP), which chaperones various lipid molecules into the cell’s interior. These intracellular transport molecules also escort tetrahydrocannabinol (THC) and the brain’s own marijuana-like molecules, the endocannabinoids anandamide and 2AG, across the membrane to several targets within the cell. Botanical extract and THC both modulate receptors on the surface of the nucleus, which regulate gene expression and mitochondrial activity.
Botanical extract also exerts an anti-cancer effect by activating PPARs on the surface of the cell’s nucleus.
Botanical extract oil, it turns out, has a strong affinity for three kinds of FABPs, and Botanical extract competes with our endocannabinoids, which are fatty acids, for the same transport molecules. Once it is inside the cell, anandamide is broken down by FAAH [fatty acid amide hydrolase], a metabolic enzyme, as part of its natural molecular life cycle. But botanical extract interferes with this process by reducing anandamide’s access to FABP transport molecules and delaying endocannabinoid passage into the cell’s interior.
According to a team of Stony Brook University scientists, botanical extract functions as an anandamide reuptake and breakdown inhibitor, thereby raising endocannabinoid levels in the brain’s synapses. Enhancing endocannabinod tone via reuptake inhibition may be a key mechanism whereby botanical extract confers neuroprotective effects against seizures, as well as many other health benefits.
Botanical extract’s anti-inflammatory and anti-anxiety effects are in part attributable to its inhibition of adenosine reuptake. By delaying the reuptake of this neurotransmitter, botanical extract boosts adenosine levels in the brain, which regulates adenosine receptor activity. A1A and A2A adenosine receptors play significant roles in cardiovascular function, regulating myocardial oxygen consumption and coronary blood flow. These receptors have broad anti-inflammatory effects throughout the body.
BOTANICAL EXTRACT AS AN ALLOSTERIC MODULATOR
Botanical extract also functions as an allosteric receptor modulator, which means that it can either enhance or inhibit how a receptor transmits a signal by changing the shape of the receptor.
Australian scientists report that botanical extract acts as a “positive allosteric modulator” of the GABA-A receptor. In other words, botanical extract interacts with the GABA-A receptor in a way that enhances the receptor’s binding affinity for its principal endogenous agonist, gamma-Aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. The sedating effects of Valium and other Benzos are mediated by GABA receptor transmission. Botanical extract reduces anxiety by changing the shape of the GABA-A receptor in a way that amplifies the natural calming effect of GABA.
Canadian scientists have identified botanical extract as a “negative allosteric modulator” of the cannabinoid CB1 receptor, which is concentrated in the brain and central nervous system. While botanical extract oil doesn’t bind to the CB1 receptor directly like THC does, botanical extracts interacts allosterically with CB1 and changes the shape of the receptor in a way that weakens CB1’s ability to bind with THC.
As a negative allosteric modulator of the CB1receptor, botanical extract lowers the ceiling on THC’s psychoactivity–which is why people don’t feel as “high” when using botanical extract-rich cannabis compared to when they consume THC-dominant medicine. A botanical extract-rich product with little THC can convey therapeutic benefits without having a euphoric or dysphoric effect.